Methylation and expression of RUNX3 gene promoter in papillary thyroid carcinoma
10.3760/cma.j.issn.1673-0860.2011.05.015
- VernacularTitle:甲状腺乳头状癌中RUNX3基因启动子甲基化及其蛋白表达的研究
- Author:
De-Tao YIN
1
;
Sheng-Li CAO
;
Yong-Fei WANG
;
Hong-Qiang LI
;
Yu-Bing ZHOU
;
Li-Yun ZHENG
;
Jin-Hua JIANG
;
Qing-Duan WANG
Author Information
1. 郑州大学附属第一医院
- Keywords:
Methylation;
Immunohistochemistry;
Core binding factor alpha 3 subunit;
Thyroid neoplasm;
Carcinoma,papillary
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(5):417-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between status of methylation of human runt-related transcription factor 3 ( RUNX3 ) gene promoter in papillary thyroid carcinoma ( PTC). Methods Methylation-specific PCR and immunohistochemical SP technique were used to detect the methylation of RUNX3 gene promoter and expression of its protein in 56 cases of PTC and their matched adjacent non-carceious epithelium(NCE). Results In NCE, there was no methylation of RUNX3 gene promoter, while in PTC the methylation rate was 35.1% (20/56), which was related to the tumor TNM stage, pathological grade and lymph node metastasis (P<0. 05). The positive rates of RUNX3 protein expression in NCE and PTC were 100. 0% and 60. 7% , respectively, with a significant difference ( χ2 = 27. 378, P < 0. 05). In PTC, the positive rates of RUNX3 protein expression in grade I and grade II were 70.0% and 37.5% , respectively ( P < 0. 05 ); the rates were 46.7% and 76.9% in lymph node metastasis group and no metastasis group, respectively ( P < 0. 05 ). Moreover, there was a distinct correlation between methylation of RUNX3 gene promoter and expression of its protein (χ 2= 21. 62, P < 0. 01). Conclusions Methylation of promoter might be one of the important factors of inactivation of RUNX3 gene, and might play an important role in carcinogenesis and progression of PTC.
