Effects of celecoxib combined with radiotherapy on apoptosis of CNE-2Z cell lines
10.3760/cma.j.issn.1673-0860.2011.07.010
- VernacularTitle:塞来昔布联合放射对鼻咽癌细胞株凋亡的影响
- Author:
Yin-Zhou XIANG
1
;
Lian-Zhi WEI
;
Lin YU
;
Ping GUI
;
Ping PENG
;
Zhi-Jie XIONG
Author Information
1. 三峡大学人民医院,湖北宜昌市第一人民医院
- Keywords:
Sulfonamides;
Pyrazoles;
Nasopharyngeal neoplasms;
Tumor cells,cultured;
Radiation tolerance;
Apoptosis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(7):572-575
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of celecoxib combined with radiotherapy on apoptosis of CNE-2Z cell lines and the potential mechanisms.Methods Four groups were used,a control,celecoxib (25 μmol/L celecoxib) ,irradiation ( 8 Gy X ray) and celecoxib plus irradiation.The radiosensitising effect was detected by clone formation experiment.Flow cytometry was used to detect the apoptosis rate of cells.The expressions of Bcl-2 and Bax were assessed by immunocytochemistry.Western blot was used to examine the expression of Caspase-3.Results Celecoxib enhanced the radiosensitivity of CNE-2Z cells.In experimental group,the mean surviving fraction and the mean lethal dose of CNE-2Z cells were 0.50 and 2.36 respectively.Compared with the irradiated group,there was significant differences between the two groups (P ＜ 0.01 ).Celecoxib combined with radiotherapy up-regulation the expression of Bax.The score of the expression of Bax in the control group and the experimental group were 1.221 ±0.116 and 2.758 ±0.256 respectively.Celecoxib combined with radiotherapy could inhibit the expression of the protein of Bcl-2.The score of the expression of Bcl-2 in the control group and the experimental group were 2.559 ± 0.144 and 1.253 ± 0.114 respectively,with significant differences ( P ＜ 0.01 ).Celecoxib combined with radiotherapy cound increase the apoptosis rate of tumor cells with significant differences ( F =7.63,P ＜ 0.01 ).Western blot showed that the expression of Caspase-3 was strengthened.Conclusion Celecoxib combined with radiotherapy could induce apoptosis and enhance the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cell lines.
