The induced apoptosis of recombinant adenovirus carrying melittin gene for hepatocellular carcinoma cell.
- Author:
Bai LI
1
;
Chang-quan LING
;
Chen ZHANG
;
Wei GU
;
Shao-xiang LI
;
Xue-qiang HUANG
;
Ya-ni ZHANG
;
Chao-qin YU
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Apoptosis; drug effects; Carcinoma, Hepatocellular; pathology; Cell Line, Tumor; Gene Expression; drug effects; Gene Silencing; drug effects; Genetic Therapy; Genetic Vectors; genetics; Humans; Liver Neoplasms; pathology; Melitten; biosynthesis; genetics; pharmacology; Recombinant Fusion Proteins; biosynthesis; genetics; Transcription, Genetic; drug effects; Transfection
- From: Chinese Journal of Hepatology 2004;12(8):453-455
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the induced apoptosis of recombinant adenovirus carrying melittin gene (Ad-rAFP-Mel) for hepatocellular carcinoma cell line (BEL-7402).
METHODSThe morphological observe, DNA electrophoresis, TUNEL and Flow cytometry assay were used to study the apoptosis of BEL-7042 cell line transfected by Ad-rAFP-Mel.
RESULTSThe morphological changes and apoptosis of BEL-7402 transfected by Ad-rAFP-Mel were confirmed with microscopy and DNA electrophoresis, TUNEL, Flow cytometry assay. The DNA ladder could be demonstrated on DNA electrophoresis in Ad-rAFP-Mel group. The apoptosis rates of BEL-7402 cells in Ad-rAFP-Mel, Ad-rAFP, and control groups were (21.5+/-2.4)%, (10.5+/-4.4)% and (3.0+/-1.4)% respectively by TUNEL assay (F = 38.0, P < 0.05) and were (7.3+/-0.5)%, (3.9+/-0.1)% and (0.8+/-0.1)% respectively by flow cytometry assay (F = 415.1, P < 0.05).
CONCLUSIONIt seems that melittin inducing apoptosis might be one of the antitumor mechanisms.
