Establishing a new genotyping method of hepatitis B virus by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) to analysis on S region and its application.
- Author:
Liang PENG
1
;
Jing-Juan DING
;
Li-Sha ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Genotype; Hepatitis B; virology; Hepatitis B virus; classification; genetics; isolation & purification; Humans; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Viral Envelope Proteins; genetics
- From: Chinese Journal of Hepatology 2004;12(8):475-478
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method of genotyping HBV using Mbo I, BsTN I, BsmA I, Hpa II and investigate the relationship between genotype and clinical spectrum of hepatitis B.
METHODS124 full-genomic HBV sequences and 13 S-genomic sequences were analyzed, genotype specific regions were identified by the restriction enzymes Mbo I, BsTN I, BsmA I, Hpa II. And 176 samples from different kinds of hepatitis B were genotyped by this method. Five samples had been randomly selected and directly sequenced their S gene, to assess the accuracy.
RESULTSIn 176 serum samples of patients with hepatitis B from Guizhou area, genotype B and C were found in 56.8% and 43.2% respectively. The proportions of genotype B and C in ASC were 40.0% and 15.7% (chi-square = 12.16, P < 0.005); and they were 31.6% and 14.0% in CHB (chi-square = 7.88, P < 0.005).
CONCLUSIONGenotyping HBV, based on S gene RFLP seems to be highly sensitive, differential and accurate and could be used in large-scale surveys. HBV genotype B and C are existed in Guizhou area.
