Inhibition of hepatitis B virus replication by RNA interference in vitro.
- Author:
Cai ZHU
1
;
Xue-Gong FAN
;
Ning LI
;
Ruo-Su YING
Author Information
- Publication Type:Journal Article
- MeSH: Hepatitis B virus; genetics; physiology; Humans; Liver Neoplasms; virology; RNA Interference; Tumor Cells, Cultured; Virus Replication; genetics
- From: Chinese Journal of Hepatology 2004;12(9):522-525
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo design pSilencer3.1-H1hygro plasmid expressing short interfering RNAs (siRNA) that targets HBV core gene region, and to evaluate inhibitory effect of this siRNA on HBV in vitro.
METHODSHepG2 2.2.15 was used as target cells. The plasmid and liposome metafectene were cotransfected into the cultured cells, HBV DNA were analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR.
RESULTSThe plasmid expressing siRNA was successfully constructed. The two constructed siRNAs could effectively inhibit HBV replication, and their inhibitive effect on HBV was dose-dependent.
CONCLUSIONThese results showed that siRNA could substantially inhibit HBV replication in the infected cells
