The anti-cancer effect of siRNA targeting human telomerase reverse transcriptase in SMMC-7721 cells.
- Author:
Su-Jun ZHENG
1
;
Yun XIA
;
Hong REN
;
Sen ZHONG
;
Ying YANG
;
Peng TAO
;
Sheng-Qi WANG
Author Information
- Publication Type:Journal Article
- MeSH: DNA-Binding Proteins; antagonists & inhibitors; genetics; Gene Targeting; Genetic Therapy; Humans; Liver Neoplasms; pathology; therapy; RNA, Small Interfering; genetics; Telomerase; antagonists & inhibitors; genetics
- From: Chinese Journal of Hepatology 2004;12(9):530-533
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the anti-cancer effects of siRNAs targeting hTERT in SMMC-7721 cells.
METHODSTwo siRNAs targeting hTERT mRNA were designed and synthesized by T7 transcription system in vitro. MMT, RT-PCR and Western blot were applied to evaluate effects on inhibiting cell growth, hTERT mRNA and protein expression in SMMC-7721 cells.
RESULTSsiRNAs decreased cell proliferation in a dose-dependent manner. At a concentration of 100 nmol/L, siRNAs exhibited obvious effects on inhibiting hTERT mRNA and protein expression in SMMC-7721 cells.
CONCLUSIONsiRNAs targeting hTERT have significant inhibitory effects on hTERT gene expression in SMMC-7721 cells. siRNA has the possibility to become a new anti-cancer agent
