The Expression of TGF-beta Isoform mRNA in the Cataract Lens Epithelial Cell.
- Author:
Do Hyung KIM
1
;
Jae Wook YANG
;
Seh Kwang PARK
;
Sung Doo KIM
;
Jun HUR
;
Ill Han YOON
;
In Hak CHOI
Author Information
1. Department of Ophthalmology, College of Medicine, Inje University, Busan, Korea. eyeyang@inje.ac.kr
- Publication Type:Original Article
- Keywords:
Cataract lens epithelial cell;
Senile cataract;
TGF-beta isoform mRNA
- MeSH:
Cataract*;
Diabetes Mellitus;
Epithelial Cells*;
Humans;
Lens, Crystalline;
Polymerase Chain Reaction;
Protein Isoforms;
RNA, Messenger*;
Transforming Growth Factor beta*;
Transforming Growth Factor beta1;
Transforming Growth Factor beta2;
Transforming Growth Factor beta3;
Transforming Growth Factors
- From:Journal of the Korean Ophthalmological Society
2005;46(8):1393-1400
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Transforming growth factor (TGF)-beta is recognized as being associated with cataractogenesis. We quantitated the mRNA expression of TGF-beta isoforms in cataractous crystalline lens to determine the effect of the isoforms on cataractogenesis. METHODS: With lens epithelial cells from thirty eyes in thirty patients, the mRNA expressions of TGF-beta1, beta2 and beta3 were quantitated by real-time SYBR Green polymerase chain reaction and the results were compared according to cataract type and presence of diabetes mellitus. RESULTS: Each isoform mRNA of TGF-beta was expressed: TGF-beta3 in all 30 eyes, TGF-beta1 in 29 eyes (96.7%), with the exception being one diabetic senile cataract, and TGF-beta2 in 9 eyes. The amount of TGF-beta1 mRNA expression was significantly lower in the diabetic cataracts than in the non-diabetic cataracts (P=0.056). CONCLUSIONS: TGF-beta was associated with cataractogenesis. It is significant that the expression of TGF-beta2 mRNA was decreased in all cataracts. The decrease of TGF-beta1 mRNA expression was more meaningful in the diabetic cataracts than in the non-diabetic cataracts.
