The relationship between microRNA-18 and BTG2 in the carcinogenesis of hepatocellular carcinoma.
- Author:
Qiong LI
1
;
Ge WANG
;
Zhi-Min ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Proliferation; Cells, Cultured; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Hep G2 Cells; Humans; Immediate-Early Proteins; genetics; metabolism; Liver; cytology; Liver Neoplasms; genetics; metabolism; pathology; MicroRNAs; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Tumor Suppressor Proteins; genetics; metabolism
- From: Chinese Journal of Hepatology 2009;17(1):42-45
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the difference of microRNA expression between HepG2 cells and L02 cells, and to identify the target genes of microRNA-18 (miR-18).
METHODSThe differentially expressed miRNAs between HepG2 cells and L02 cells were identified by miRNA chip. Target genes of miR-18 were predicted bioinformatically. Furthermore, the expression of B-cell translocation gene 2 (BTG2), a putative target gene of miR-18, was analyzed in hepatocellular carcinoma tissues and the surrounding non-cancerous tissues by RT-PCR and northern blot.
RESULTSmiR-18 was over-expressed in HepG2 cells compared to L02 cells. Altogether 609 genes, including genes involved in cell proliferation, differentiation, apoptosis and transcriptional regulation, are identified as putative miR-18 targets. The mRNA level of BTG2 was much lower in hepatocellular carcinoma tissues than in the corresponding non-cancerous tissues.
CONCLUSIONmiR-18 is over-expressed in HepG2 cells compared to L02 cells, and it may negatively regulate the expression of BTG2, a tumor suppressor gene.
