Role of CD4-CD8- T cells in the murine hepatitis virus type 3 induced chronic viral hepatitis.
- Author:
Xiao-Jing WANG
1
;
Wei-Ming YAN
;
Jiang-Guo ZHANG
;
Hong-Wu WANG
;
Yong ZOU
;
Xiao-Ping LUO
;
Qin NING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CD4-Positive T-Lymphocytes; immunology; CD8-Positive T-Lymphocytes; immunology; Coronavirus Infections; immunology; pathology; virology; Female; Flow Cytometry; Hepatitis, Viral, Animal; immunology; pathology; virology; Liver; immunology; pathology; Mice; Mice, Inbred C3H; Murine hepatitis virus; Spleen; immunology; pathology; T-Lymphocyte Subsets; immunology; Time Factors
- From: Chinese Journal of Hepatology 2009;17(3):180-183
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate role of CD4-CD8- T cells in murine hepatitis virus type 3 (MHV-3) induced chronic viral hepatitis in C3H/Hej mice and to identify their surface markers.
METHODSThirty C3H/Hej mice received 10 Pfu MHV-3 intraperitoneally, the CD4-CD8- T cells were isolated using magnetic bead sorting on 0, 4, 15, 30, 40 days post MHV-3 infection. The cytotoxic effects of CD4-CD8- T cells on normal and infected hepatocytes, CD8+ T cells and unrelated-virus (murine cytomegalovirus, MCMV) infected CD8+ T cells were examined by non-radioactive cytotoxicity assay. The surface markers of CD4-CD8- T cells were determined by flow cytometry.
RESULTSMHV-3 infected CD4-CD8- T cells showed significant cytotoxic effect on CD8+ T cells, but not on infected hepatocytes or MCMV infected CD8+ T cells. The analysis of cell surface markers demonstrated that the CD4-CD8- T cells are a completely new T cell subset.
CONCLUSIONSCD4-CD8- T cells have significant cytotoxic effect on virus specific CD8+ T cells in MHV-3 infected C3H/Hej mice, which suggests that CD4-CD8- T cells have immune modulatory functions in the development of chronic viral hepatitis. The phenotype of these CD4-CD8- T cells detected by flow cytometry is TCR alpha beta +CD3+CD4- CD8- CD25- CD28- CD30- CD44+.
