Expression and role of augmenter of liver regeneration in patients with hepatic failure.
- Author:
Hai-Ying YU
1
;
Hai-Jun HUANG
;
Dai-Rong XIANG
;
Jun LI
;
Ji-Fang SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Case-Control Studies; Enzyme-Linked Immunosorbent Assay; Female; Gene Expression; Hepatitis B, Chronic; blood; metabolism; pathology; Hepatocytes; metabolism; Humans; Liver; metabolism; pathology; Liver Failure, Acute; blood; metabolism; pathology; Liver Regeneration; Mice; Mice, Inbred BALB C; Polymerase Chain Reaction; methods; Prognosis; Proteins; genetics; metabolism; RNA, Messenger; genetics; metabolism; Recombinant Proteins; genetics; metabolism
- From: Chinese Journal of Hepatology 2009;17(3):217-220
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and role of augmenter of liver regeneration (ALR) in hepatic failure.
METHODSALR polyclonal antibody was prepared and purified. Serum ALR in patients with hepatic failure, chronic hepatitis B and healthy persons were quantified by ELISA, ALR mRNA in hepatic tissues were quantified by real-time PCR.
RESULTSDifferent serum ALR levels foreshowed different outcomes for hepatic failure patients: The liver function was restored in 6 patients with higher ALR level [(1613.5+/-369.6) pmol/ml], and the liver function was deteriorated in 12 patients with lower ALR level [(462.3+/-235.8) pmol/ml]. ALR level in patients with chronic hepatitis B [(969.2+/-332.5) pmol/ml] was similar to that in healthy persons [(806.9+/-240.8) pmol/ml]. ALR mRNA level in hepatic failure patients receiving OLT (103.45 copies/microl) was lower than that in chronic hepatitis B patients (104.37 copies/microl) and healthy persons (104.31 copies/microl), ALR mRNA level in chronic hepatitis B and healthy persons was similar.
CONCLUSIONThese findings suggest serum ALR level reflected ALR mRNA level in liver and is helpful in estimating the survival time of patients with hepatic failure.
