Application of Ac/Ds transposon system to genetate marker gene free transgenic plants in rice.
- Author:
Wei-Zheng JIN
1
;
Rui-Jun DUAN
;
Fan ZHANG
;
Shuang-Yan CHEN
;
Yun-Rong WU
;
Ping WU
Author Information
1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hanrgzhou 310029, China.
- Publication Type:Journal Article
- MeSH:
Agrobacterium tumefaciens;
genetics;
Blotting, Southern;
Crosses, Genetic;
DNA Transposable Elements;
genetics;
Genetic Vectors;
genetics;
Oryza;
genetics;
Plants, Genetically Modified;
genetics;
Polymerase Chain Reaction;
Transformation, Genetic
- From:
Chinese Journal of Biotechnology
2003;19(6):668-673
- CountryChina
- Language:Chinese
-
Abstract:
It is critical to generate marker gene free transgenic plants for retransformating or eliminating the potential harmfulness of marker gene and its product. In this study, Ac/Ds transposon system was developed for removal of hpt selection marker gene to obtain marker-free transgenic plants in rice ( Oryza sativa L.). Ds element containing the interesting gene bar was constructed next to the selection marker gene hpt to get Ds-T-DNA. Rice plants were transformed by Agrobacterium tumefaciens EHA105 containing Ac-T-DNA and Ds-T-DNA respectively. Rice plant containing single copy Ac-T-DNA was crossed with plant containing single copy Ds-T-DNA to obtain the F1 plant containing both Ac and Ds elements. F1 plant was self-crossed to produce F2 progeny in which T-DNA insert and transposed Ds element segregated independently. Two plants contained Ds element but no hpt marker gene in total 100 F2 plants. The result indicated that Ac/Ds transposon system could be used as a vector system for generating marker gene free transgenic plants in rice.
