Study of recombinant stem cell factor.
- Author:
Jun WU
1
;
Xin GONG
;
Shao-Hong CHANG
;
Zhi-Hu ZHAO
;
Cong-Lin ZUO
;
Qing-Jun MA
Author Information
1. Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Differentiation;
drug effects;
Cells, Cultured;
Chromatography, High Pressure Liquid;
Haplorhini;
Hematopoietic Stem Cells;
cytology;
drug effects;
Humans;
Recombinant Proteins;
chemistry;
genetics;
metabolism;
pharmacology;
Sequence Analysis, Protein;
Spectrometry, Mass, Fast Atom Bombardment;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Stem Cell Factor;
chemistry;
genetics;
metabolism;
pharmacology
- From:
Chinese Journal of Biotechnology
2003;19(6):698-704
- CountryChina
- Language:Chinese
-
Abstract:
Stem cell factor is an important hematopoietic growth factor. In this study, the human stem cell factor was produced by recombinant E. coli, and the structure and biological activity of the recombinant stem cell factor(rhSCF) was studied. It was indicated that the rhSCF was a uncovalent dimer in phosphate buffer,and had the correct mass spectra, mass peptides spectra, composition of amino acid, N-terminal sequernce, C-terminal sequence and intrachain disulfide linkages, rhSCF alone or synergy with rhG-CSF could mobilze hematopoietic progenitors to blood in monkey.
