Enhancements in ethanol tolerance of a self-flocculating yeast by calcium ion through decrease in plasmalemma permeability.
- Author:
Chun-Keng HU
1
;
Feng-Wu BAI
;
Li-Jia AN
Author Information
1. Department of Biological Engineering, Dalian University of Technology, Dalian 116024, China.
- Publication Type:Journal Article
- MeSH:
Calcium;
pharmacology;
Cell Membrane;
drug effects;
metabolism;
Cell Membrane Permeability;
drug effects;
Ethanol;
pharmacology;
Saccharomyces cerevisiae;
drug effects;
growth & development;
metabolism;
Schizosaccharomyces;
drug effects;
growth & development;
metabolism;
Temperature
- From:
Chinese Journal of Biotechnology
2003;19(6):715-719
- CountryChina
- Language:English
-
Abstract:
Ca2+ at 1.64 mmol/L markedly increased ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae. After 9 h of exposure to 20% (V/V) ethanol at 30 degrees C , no viability remained for the control whereas 50.0% remained for the cells both grown and incubated with ethanol in Ca2+ -added medium. Furthermore, when subjected to 15% (V/V) ethanol at 30 degrees C, the equilibrium nucleotide concentration and plasma membrane permeability coefficient (P' ) of the cells both grown and incubated with ethanol in Ca2+ -added medium accounted for only 50.0% and 29.3% those of the control respectively, indicating that adding Ca2+ can markedly reduce plasma membrane permeability of yeast cells under ethanol stress as compared with the control. Meanwhile, high viability levels acquired by the addition of Ca2+ exactly corresponded to the striking decreases in extracellular nucleotide concentration and P' achieved with identical approach. Therefore, the enhancing effect of Ca2+ on ethanol tolerance of this strain is closely related to its ability to decrease plasma membrane permeability of yeast cells subjected to ethanol stress.
