Affection of metallothionein-3 to dUTPase's accommodating cellular toxicity of dUTP.
- Author:
Qiao-Lin CHEN
1
;
Qiao-Hua KANG
;
Hong-Wei REN
;
Zong-Yuan WANG
;
Bing-Gen RU
Author Information
1. Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, National Laboratory of Protein Engineering, Beijing 100871, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Deoxyuracil Nucleotides;
antagonists & inhibitors;
chemistry;
Nerve Tissue Proteins;
chemistry;
genetics;
pharmacology;
Neurons;
cytology;
drug effects;
Protein Interaction Domains and Motifs;
Pyrophosphatases;
chemistry;
genetics;
pharmacology;
Recombinant Proteins;
biosynthesis;
genetics;
pharmacology;
Transfection
- From:
Chinese Journal of Biotechnology
2004;20(3):389-393
- CountryChina
- Language:Chinese
-
Abstract:
Metallothionein-3 (MT-3), renamed as growth inhibitory factor (GIF), is a brain specific member of the metallothionein family. Human dUTPase is a recently found protein in brain that can interact with hMT-3. They have the growth inhibitory activity on neuron cell by interaction. To study the affection of hMT-3 to dUTPase's eliminating the cellular toxicity caused by dUTP, the pSVHA-dUTPase and pFLag-hMT-3 genes have been transfected into HEK293 cells. In addition, the dUTPase and hMT-3 proteins were expressed in BL21 to study the role of hMT-3 on the hydrolyzation of dUTP by dUTPase. The results demonstrate that the cells co-transfected with dUTPase and hMT-3 genes have more strong resistibility to dUTP than the cells transfected only with dUTPase gene. And that the hMT-3 protein can accelerate the hydrolyzation of dUTP by dUTPase. All these indicate that hMT-3 can cooperate with dUTPase to protect better the 293 cells from dUTP. This research offered the theoretic elements for the application of hMT-3 and dUTPase in chemic cure.
