Humoral immune response elicited by plasmid DNA containing HGV E2 gene fragment.
- Author:
Fethia Ben YEBDRI
1
;
Abderrahmane AAZAZ
;
Kai YE
;
Hui-Wen MA
;
Li-Heng TONG
Author Information
1. College of Pharmacy, Wuhan University, Wuhan 430072, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Female;
GB virus C;
immunology;
Hepatitis Antibodies;
blood;
Mice;
Mice, Inbred BALB C;
Plasmids;
Recombinant Fusion Proteins;
biosynthesis;
immunology;
Vaccines, DNA;
immunology;
Viral Envelope Proteins;
genetics;
immunology;
Viral Hepatitis Vaccines;
immunology
- From:
Chinese Journal of Biotechnology
2004;20(5):683-688
- CountryChina
- Language:English
-
Abstract:
In order to study the feasibility of E2 gene fragment of hepatitis virus G(HGV) as a component of DNA vaccine against the hepatitis virus G infection, a 559bp DNA fragment encoding HGV E2 was cloned into plasmid pCMV-S from pThioHis-E2 in the same reading frame with HBsAg gene to form a recombinant plasmid named pCMV-S-E2. BALB/c mice of Kunming strain were immunized with purified plasmid DNA of pCMV-S-E2 by intra-muscularly inoculation. The immunizations were boosted twice at an interval of 14 days. The whole blood was collected from mice orbit on the day-8 after the last boost. Mice sera were screened by ELISA to determine the humoral immune response using E2-GST fusion protein as the immobilized antigen and the sera from mice immunized with pCMV-S as control. The result indicated that the immunization with plasmid DNA of pCMV-S-E2 could induce quite strong humoral immune response.
