Expression patterns of PPARgamma2 in differentiation of mouse embryonic stem (ES) cells into adipocytes.
- Author:
Cheng-Jian LI
1
;
Xiao-Lan ZHANG
;
Yang YANG
;
Chong-Ben ZHANG
Author Information
1. College of Life Sciences, Peking University, Beijing 100871, China.
- Publication Type:Journal Article
- MeSH:
Adipocytes;
cytology;
Animals;
Cell Differentiation;
genetics;
Cells, Cultured;
Electroporation;
Embryonic Stem Cells;
cytology;
metabolism;
Mice;
PPAR gamma;
biosynthesis;
genetics;
Promoter Regions, Genetic;
genetics;
Transfection
- From:
Chinese Journal of Biotechnology
2005;21(2):187-191
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression patterns of peroxisome proliferator activated receptor2 (PPARgamma2) gene in the differentiation of mouse embryonic stem (ES) cells into adipocytes, mouse ES cells were transfected with the vector of pPPARgamma2-promoter-luciferase, and PPARgamma2 expressions were analyzed by detecting luciferase activities and by detecting the protein expressions using western blotting. The results showed that the gene PPARgamma2 did not express in undifferentiated mouse ES cells and in embryoid bodies (EBs) within the first 2 days of differentiation induction after EB formation, and began to express from the third day of differentiation induction after EB formation to the finish of the differentiation. The gene's expression in differentiated adipocytes was much stronger than that in differentiating preadipocytes. In Conclusion our results reported for the first time the five-step expression patterns of the gene PPARgamma2 during the whole differentiation procedures from mouse ES cells into adipocytes via preadipocytes, and supported the previous studies that PPARgamma2 is a fat-specific gene that expresses only in developed and developing adipose tissues.
