Study on expression of genes in Tamarix androssowii under NaHCO3 stress using gene chip technology.
- Author:
Chuan-Ping YANG
1
;
Yu-Cheng WANG
;
Gui-Feng LIU
;
Jing JIANG
;
Guo-Dong ZHANG
Author Information
1. College of Forestry, Northeast Forestry University, Harbin 150040, China.
- Publication Type:Journal Article
- MeSH:
DNA, Complementary;
genetics;
Gene Expression Profiling;
methods;
Genes, Plant;
genetics;
Oligonucleotide Array Sequence Analysis;
Sodium Bicarbonate;
pharmacology;
Stress, Physiological;
Tamaricaceae;
drug effects;
genetics
- From:
Chinese Journal of Biotechnology
2005;21(2):220-226
- CountryChina
- Language:Chinese
-
Abstract:
Gene chip technology was employed to study gene expression in Tamarix androssowii under NaHCO3 stress. cDNAs from T. androssowii treated with NaHCO3 solution and that from control group were labeled with fluorescent dye CyS and Cy3 respectively. The two fluorescent cDNA probes were mixed and hybridized to gene chips containing T. androssowii genes, and the chips were scanned using biochip scanning system. Differential expression of genes was analyzed through calculation of the ratio of Cy5 to Cy3 signal intensities. Total of 89 genes differentially expressed were identified, among them, 27 showed down regulated expression and 62 showed up regulated expression. Blastx analysis showed that the function of the differentially expressed genes could be grouped into some categorizations such as photosynthesis, reactive oxygen species eliminated, regulation of osmotic potential, regulation of gene expression and signal transduction, metabolism, development, ribosomal protein, protein breakdown and recycling, transporter, water channel proteins and so on. Based on this research, some function-unknown or novel unreported genes that respond to salt stress were also identified, and these genes may have important functions in salt resistance of T. androssowii. Some important pathways of salt resistance in T. androssowii are revealed, and the gene expression profiling of T. androssowii under salt stress and without stress is obtained in this study.
