Establishment and optimization of the regeneration system for common dandelion (Taraxacum officinale Weber).
- Author:
Hua CHEN
1
;
Ping LI
;
Jing LIU
;
Yin-Xin LI
Author Information
1. Key Laboratory of Phytosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093.
- Publication Type:Journal Article
- MeSH:
Culture Media;
Germination;
physiology;
Plant Leaves;
growth & development;
Regeneration;
Taraxacum;
growth & development;
Tissue Culture Techniques;
methods
- From:
Chinese Journal of Biotechnology
2005;21(2):244-249
- CountryChina
- Language:Chinese
-
Abstract:
A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Multiple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For indirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerated plantlets were obtained when these calli were cultured on MS medium containing 1.0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific bands.
