Anticlastogenic effect of redistilled cow's urine distillate in human peripheral lymphocytes challenged with manganese dioxide and hexavalent chromium.
- Author:
Dipanwita DUTTA
1
;
S Saravana DEVI
;
K KRISHNAMURTHI
;
T CHAKRABARTI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antimutagenic Agents; pharmacology; Antioxidants; pharmacology; Cattle; urine; Cells, Cultured; Chromium; antagonists & inhibitors; toxicity; DNA Damage; Humans; Hydrogen-Ion Concentration; Lymphocytes; drug effects; Manganese Compounds; antagonists & inhibitors; Mutagenicity Tests; Mutagens; toxicity; Oxides; antagonists & inhibitors; toxicity; Urine; chemistry
- From: Biomedical and Environmental Sciences 2006;19(6):487-494
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the anticlastogenic effect of redistilled cow's urine distillate (RCUD) in human peripheral lymphocytes (HLC) challenged with manganese dioxide and hexavalent chromium.
METHODSThe anticlastogenic activity of redistilled cow's urine distillate was studied in human polymorphonuclear leukocytes (HPNLs) and human peripheral lymphocytes in vitro challenged with manganese dioxide and hexavalent chromium as established genotoxicants and clastogens which could cause induction of DNA strand break, chromosomal aberration and micronucleus. Three different levels of RCUD: 1 microL/mL, 50 microL/mL and 100 microL/mL, were used in the study.
RESULTSManganese dioxide and hexavalent chromium caused statistically significant DNA strand break, chromosomal aberration and micronucleus formation, which could be protected by redistilled cow's urine distillate.
CONCLUSIONThe redistilled cow's urine distillate posseses strong antigenotoxic and anticlastogenic properties against HPNLs and HLC treated with Cr+6 and MnO2. This property is mainly due to the antioxidants present in RCUD.
