Action of Schwann cells implanted in cerebral hemorrhage lesion.
- Author:
Hong WAN
1
;
Shao-Dong ZHANG
;
Jun-Hua LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cerebral Hemorrhage; metabolism; therapy; GAP-43 Protein; metabolism; Rats; Rats, Wistar; Schwann Cells; metabolism; transplantation; Sciatic Nerve; cytology; embryology
- From: Biomedical and Environmental Sciences 2007;20(1):47-51
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate whether there is neogenesis of myelin sheath and neuron after transplantation of Schwann cells into cerebral hemorrhage lesion.
METHODSSchwann cells were expanded, labeled with BrdU in vitro and transplanted into rat cerebral hemorrhage with blood extracted from femoral artery and then injected into the basal nuclei. Double immunohistochemistry staining and electron microscopy were used to detect the expression of BrdU/MBP and BrdU/GAP-43 and remyelination.
RESULTSBrdU/MBP double positive cells could be seen at 1 week up to 16 weeks after transplantation of Schwann cells. Thin remyelination was observed under electron microscope. GAP-43 positive cells appeared after 12 weeks and were found more in Hippocamp.
CONCLUSIONSGrafted Schwann cells participate in remyelination and promoter nerve restore in rat cerebral hemorrhage.
