Cloning and sequence analysis of the full-length cDNA of a novel yp05 gene associated with citrinin production in Monascus aurantiacus.
- Author:
Yong-Hua XIONG
1
;
Yang XU
;
Wei-Hua LAI
;
Yan-Pin LI
;
Hua WEI
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; Blotting, Northern; Citrinin; biosynthesis; Cloning, Molecular; DNA, Complementary; chemistry; Fungal Proteins; chemistry; genetics; Gene Library; Molecular Sequence Data; Monascus; genetics; metabolism; Mycelium; genetics; metabolism; Pigments, Biological; biosynthesis; RNA, Messenger; metabolism; Sequence Alignment; Sequence Analysis, DNA
- From: Biomedical and Environmental Sciences 2007;20(2):135-140
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo obtain the full-length cDNA of a novel gene (named yp05) associated with citrinin production-related genes in Monascus aurantiacus.
METHODSTotal RNA was extracted from mycelium, 3' and 5' cDNA end of yp05 gene was amplified using smart trace cDNA amplification kit, and the full-length cDNA of a novel gene (named yp05) was obtained from the electronic assembly of 3'-RACE and 5'-RACE products.
RESULTSThis yp05 gene was 787 bp including a 597 bp open reading frame (ORF) and encoded a deduced protein with 199 amino acid residues, and the amino acid sequence of this protein was found similar with the sequences of many fungal manganese-superoxide dismutases in the GenBank with the aid of BLASTp. The transcription of yp05 gene in Monascus strains was analyzed with the aid of Northern blotting. The transcription of yp05 gene was only detected in Monascus strains, provided that citrinin was produced.
CONCLUSIONThe transcription of yp05 gene belongs to differential expression genes of citrinin yielded from Monascus and has no correlation with the biosynthesis pathway of red pigments.
