Biodegradability of chlorinated anilines in waters.
- Author:
Chao WANG
1
;
Guang-Hua LU
;
Yan-Jie ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Aniline Compounds; metabolism; pharmacology; Bacteria; drug effects; growth & development; metabolism; Biodegradation, Environmental; Rivers; chemistry; microbiology; Water Pollutants, Chemical; metabolism; pharmacology; Water Purification
- From: Biomedical and Environmental Sciences 2007;20(2):141-145
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo identify the bacteria tolerating chlorinated anilines and to study the biodegradability of o-chloroaniline and its coexistent compounds.
METHODSMicrobial community of complex bacteria was identified by plate culture observation techniques and Gram stain method. Bacterial growth inhibition test was used to determine t he tolerance of complex bacteria to toxicant. Biodegradability of chlorinated anilines was determined using domesticated complex bacteria as an inoculum by shaking-flask test.
RESULTSThe complex bacteria were identified, consisting of Xanthomonas, Bacillus alcaligenes, Acinetobacter Pseudomonas, and Actinomycetaceae nocardia. The obtained complex bacteria were more tolerant to o-chloroaniline than mixture bacteria in natural river waters. The effects of exposure concentration and inoculum size on the biodegradability of o-chloroaniline were analyzed, and the biodegradation characteristics of single o-chloroaniline and 2, 4-dichloroaniline were compared with the coexistent compounds.
CONCLUSIONThe biodegradation rates can be improved by decreasing concentration of compounds and increasing inoculum size of complex bacteria. When o-chloroaniline coexists with aniline, the latter is biodegraded prior to the former, and as a consequence the metabolic efficiency of o-chloroaniline is improved with the increase of aniline concentration. Meanwhile, when o-chloroaniline coexists with 2,4-dichloroaniline, the metabolic efficiency of 2,4-dichloroaniline is markedly improved.