Inhibitory effects of selenium on telomerase activity and hTERT expression in cadmium-transformed 16HBE cells.
- Author:
Hua-Jie CHEN
1
;
Ri-An YU
;
Ling-Fei HE
;
She-Juan AN
;
Zhi-Gang WU
;
Ke-Di YANG
;
Xue-Min CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Cadmium; pharmacology; Cell Line, Transformed; DNA Primers; Humans; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction; Sodium Selenite; pharmacology; Telomerase; antagonists & inhibitors; genetics
- From: Biomedical and Environmental Sciences 2007;20(4):307-312
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of sodium selenite on telomerase activity and expression of hTERT mRNA in cadmium-transformed 16HBE cells.
METHODSTelomerase activity and expression of genes were measured after cultured cadmium-transformed 16HBE cells were exposed to sodium selenite at different doses (0.625, 1.25, 2.50, 5.00 micromol/L) for 24 hours.
RESULTSSelenium decreased telomerase activity in cadmium-transformed 16HBE cells. There existed an obvious dose-effect relationship between the selenium concentration and these changes. The expression of hTERT and c-myc mRNA also decreased but the expression of mad1 mRNA increased after exposure to selenium for 24 hours. No difference was found in expression of hTRF1 and hTRF2 mRNA after incubated with sodium selenite for 24 hours, compared with control group.
CONCLUSIONSelenium inhibits telomerase activity by decreasing hTERT and c-myc mRNA expression and increasing mad1 mRNA expression in cadmium-transformed 16HBE cells and selenium concentration is significantly correlated with these changes.
