Effect of X-rays on expression of caspase-3 and p53 in EL-4 cells and its biological implications.
- Author:
Gui-Zhi JU
1
;
Bo SHEN
;
Shi-Long SUN
;
Feng-Qin YAN
;
Shi-Bo FU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Caspase 3; metabolism; radiation effects; Caspases; metabolism; radiation effects; Cell Line, Tumor; Mice; Tumor Suppressor Protein p53; metabolism; radiation effects; X-Rays
- From: Biomedical and Environmental Sciences 2007;20(6):456-459
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effect of X-rays on expression of caspase-3 and p53 protein in EL-4 cells and its implications in induction of apoptosis and polyploid cells.
METHODSMouse lymphoma cell line (EL-4 cells) was used. Fluorescent staining and flow cytometry analysis were employed for measurement of protein expression, apoptosis, cell cycle, and polyploid cells.
RESULTSThe expression of caspase-3 protein increased significantly at 8 h and 12 h, compared with that of sham-irradiated control (P<0.05, respectively) and the expression of p53 protein increased significantly at 2, 4, 8, 12, and 24 h, compared with that of sham-irradiated control (P<0.05-P<0.01) in EL-4 cells after 4.0 Gy X-irradiation. Apoptosis of EL-4 cells was increased significantly at 2, 4, 8, 12, 24, 48, and 72 h after 4.0 Gy exposure, compared with that of sham-irradiated control (P<0.05-P<0.001). G2 phase cells were increased significantly at 4, 8, 12, 24, 48, and 72 h (P<0.05-P<0.001). However, no marked change in the number of 8 C polyploid cells was found from 2 to 48 h after 4.0 Gy exposure.
CONCLUSIONThe expressions of caspase-3 and p53 protein in EL-4 cells are induced by X-rays, which might play an important role in the induction of apoptosis, and the molecular pathway for polyploid formation might be p53-independent.
