Analysis of gene expression of fatty acid synthase in squamous cell carcinoma of the oral cavity by the method of real-time quantitative PCR.
- Author:
Yu-feng WANG
1
;
Xiao-yi WANG
;
Qing-hong GAO
;
Xiao-yong YANG
;
Hong-ping WANG
;
Lei ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; Fatty Acid Synthases; Humans; Mouth Neoplasms; Polymerase Chain Reaction
- From: West China Journal of Stomatology 2006;24(6):551-554
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo relatively quantify the gene expression of fatty acid synthase in squamous cell carcinoma, adjacent tissue, and some normal oral tissues by real-time quantitative PCR.
METHODSThe tissues were collected fresh from surgical specimens. The collected tissues were minced. Then the total RNA was extracted. The RNA was reversely transcripted into cDNA with random prime. And then the cDNA was amplified by real-time quantitative PCR to quantify the gene expression of FAS according to an internal control GAPDH. The difference of FAS gene expression was compared between squamous cell carcinoma, adjacent tissue, and some normal oral tissues.
RESULTSThe expression of FAS of squamous cell carcinoma was notably higher than the other two (P < 0.001).
CONCLUSIONReal-time quantitative PCR provides a method for monitoring the expression of fatty acid synthetic activity in squamous cell carcinoma, adjacent and normal tissues.
