Cloning and characterization of genes differentially expressed in human dental pulp cells and gingival fibroblasts.
- Author:
Zhong-dong WANG
1
;
Ji-nan WU
;
Lin ZHOU
;
Jun-qi LING
;
Xi-min GUO
;
Ming-zhen XIAO
;
Feng ZHU
;
Qin PU
;
Yu-bo CHAI
;
Zhong-liang ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Cloning, Molecular; Cloning, Organism; Dental Pulp; Fibroblasts; Gene Library; Gingiva; Humans; Polymerase Chain Reaction
- From: West China Journal of Stomatology 2007;25(1):75-78
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the biological properties of human dental pulp cells (HDPC) by cloning and analysis of genes differentially expressed in HDPC in comparison with human gingival fibroblasts (HGF).
METHODSHDPC and HGF were cultured and identified by immunocytochemistry. HPDC and HGF subtractive cDNA library was established by PCR-based modified subtractive hybridization, genes differentially expressed by HPDC were cloned, sequenced and compared to find homogeneous sequence in GenBank by BLAST.
RESULTSCloning and sequencing analysis indicate 12 genes differentially expressed were obtained, in which two were unknown genes. Among the 10 known genes, 4 were related to signal transduction, 2 were related to trans-membrane transportation (both cell membrane and nuclear membrane), and 2 were related to RNA splicing mechanisms.
CONCLUSIONThe biological properties of HPDC are determined by the differential expression of some genes and the growth and differentiation of HPDC are associated to the dynamic protein synthesis and secretion activities of the cell.
