Influential factor analysis and improvement of cultural method of oral mucosal epithelial cells.
- Author:
Xiang WANG
1
;
Wen-xin GAO
;
Li WANG
;
Xiu-feng WEI
;
Ying-xin CHEN
;
Xiao-feng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Culture Techniques; Epithelial Cells; Epithelium; Factor Analysis, Statistical; Humans; Keratinocytes; Mouth Mucosa
- From: West China Journal of Stomatology 2007;25(1):79-82
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze influential factors of cultural method of human oral mucosal epithelial cells (hOMEC)and to establish a reliable cell culture system for hOMEC.
METHODSBenzalkonium bromide and gentamicin sulfate were used to prevent microbial contamination. Separations of epithelium from underlying connective tissues with Dispase at different concentration were compared. Enzyme digestion was used to isolate cells and keratinocyte-serum free medium(K-SFM) was employed for primary culture and subculture of hOMEC.
RESULTSMicrobial contamination was under control. Separation of epithelium from underlying connective tissues with 0.40% Dispase was more complete than that of 0.25% Dispase. The cells grew fast and well in vitro.
CONCLUSIONThe high successful culture of hOMEC and simplified procedures could be obtained with improvement of methods.
