Investigation of the degradation and the mechanisms of artificial basement membrane co-cultured with oral carcinoma-associated fibroblasts.

Author: Hui-yun SONG ¹ ; Xin HE ; Hong-mei ZHOU ; Sheng-fu LI ; Chun-hua FU ; Dan LONG
Author Information

1. Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Publication Type:Journal Article
MeSH: Basement Membrane; Coculture Techniques; Enzyme Precursors; Fibroblasts; Gelatinases; Humans; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Membranes, Artificial; Mouth Neoplasms
From: West China Journal of Stomatology 2007;25(3):223-225
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the degradation of artificial basement membrane (matrigel) co-cultured with oral carcinoma-associated fibroblasts (CAFs) and its possible mechanism.
METHODSCAFs and normal fibroblasts (NFs) were incubated on matrigel for 24, 48, 72 h. Equivalent amounts of conditioned medium were collected and assayed for total protein, hydroxyproline and matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) activity by gelatin zymography.
RESULTSOral CAFs were superior to oral NFs in total protein and hydroxyproline density, CAFs present more pro-MMP-2 and activated MMP-2.
CONCLUSIONCAFs were superior to NFs in degradation of matrigel. CAFs might play a key role in the reconstitution of extracellular matrix and the progression of tumor.