Synthetic and photo-immobilization of photo-active tumor necrosis factor-alpha.
- Author:
Yanqin GUAN
1
;
Huizhou ZHONG
;
Xiaochun WANG
;
Tianhong ZHOU
Author Information
1. Biotechnology Research Institute, College of Life Science, South China Normal University, Guangzhou 510631, China. gyq69@163.com
- Publication Type:Journal Article
- MeSH:
Female;
Humans;
Photochemistry;
Tumor Necrosis Factor-alpha;
chemistry;
therapeutic use;
Ultraviolet Rays;
Uterine Cervical Neoplasms;
therapy
- From:
Journal of Biomedical Engineering
2006;23(2):346-349
- CountryChina
- Language:Chinese
-
Abstract:
Tumor Necrosis Factor is one of the cell factors with much stronger anti-tumor activity. In this study, photoactive arylazide-4-azidobenzoic acid was modified to Tumor Necrosis Factor-alpha (TNF-alpha). The IR data (2127cm(-1)) was given to confirm the modification. By photo-immobilization, this modified TNF-a was immobilized on cell culture polystyrene. Both the relation between the amount of TNF-alpha in feed and immobilized TNF-alpha and the influence of different UV irradiation time on photo-immobilization are discussed. Microscopic observations of the photo-immobilization TNF-alpha were made by use of scanning electrom microscope and atomic force microscope.
