1,25-dihydroxyvitamin D3 pretreatment inhibits house dust mite-induced thymic stromal lymphopoietin release by human airway epithelial cells.
- Author:
Liqin ZHOU
1
;
Hangming DONG
;
Haijin ZHAO
;
Mengchen ZOU
;
Lihong YAO
;
Fei ZOU
;
Shaoxi CAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bronchi; cytology; Calcitriol; pharmacology; Cell Line; Cytokines; metabolism; Epithelial Cells; drug effects; metabolism; Humans; Pyroglyphidae
- From: Journal of Southern Medical University 2014;34(4):492-496
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of 1,25-dihydroxyvitamin D3 (1,25VD3) on house dust mites (HDM)-induced expression of thymic stromal lymphopoietin (TSLP) in human airway epithelial cells in vitro.
METHODSHuman airway epithelial 16HBE cells were incubated with 200, 400, and 800 U/L in the absence or presence of 1,25VD3 (10(-8) mol/L) for 6 h and 24 h, and TSLP mRNA and protein expressions in the cells were assessed using quantitative PCR and ELISA.
RESULTS16HBE cells incubated with HDM at 200, 400, and 800 U/L showed significantly increased TSLP mRNA and protein expressions (P<0.05). Pretreatment of the cells with 1,25VD3 obviously lowered 400 U/L HDM-induced TSLP expressions (P<0.05), but 1,25VD3 added along with HDM in the cells did not produce significant effects on TSLP expressions (P=0.58).
CONCLUSIONBoth 1,25VD3 and HDM can induce TSLP expression and release in 16HBE cells, but pretreatment with 1,25VD3 can decrease HDM-augmented TSLP expression in the cells.
