Effect of exogenous hydrogen sulfide on BACE-1 enzyme expression and β-amyloid peptide metabolism in high-glucose primary neuronal culture.
- Author:
Lijuan ZHU
1
;
Xiaoshan CHEN
;
Xuanli HE
;
Yunwen QI
;
Yong YAN
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid Precursor Protein Secretases; metabolism; Amyloid beta-Peptides; metabolism; Animals; Aspartic Acid Endopeptidases; metabolism; Cells, Cultured; Culture Media; chemistry; Glucose; chemistry; Hydrogen Sulfide; pharmacology; Neurons; drug effects; metabolism; Peptide Fragments; metabolism; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2014;34(4):504-510
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of exogenous hydrogen sulfide (H2S) on β-site APP cleaving enzyme 1 (BACE-1) and β-amyloid peptide (Aβ) metabolism in primary culture of neurons under high-glucose condition.
METHODSThe cortical neurons in primary culture under normal and high glucose (60 mmol/L) conditions for 24 h were exposed to 25, 50 and 100 µmol/L NaHS. Aβ1-42 concentration in the cell culture was measured by ELISA, and BACE-1 mRNA and protein levels were detected by fluorescent quantitative real-time PCR and Western blotting, respectively.
RESULTSCompared with the neurons cultured in normal glucose, the neurons exposed to high glucose showed significantly increased Aβ1-42 concentration and BACE-1 mRNA and protein expressions (P<0.05). Exposure to 25, 50 and 100 µmol/L NaHS significantly decreased Aβ1-42 concentration and BACE-1 mRNA and protein expressions in the high-glucose cell culture (P<0.05).
CONCLUSIONNeurons exposed to high glucose exhibit increased Aβ1-42 levels and BACE-1 mRNA and protein expressions, which can be concentration-dependently decreased by NaHS.
