SCF/c-Kit signaling promotes invasion of T24 cells via PI3K pathway.
- Author:
Shujun GUO
1
;
Xiangnan TAO
;
Yimeng WANG
;
Jie TANG
;
Lin SHEN
;
Chuanwang SONG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Transitional Cell; metabolism; pathology; Cell Line, Tumor; Humans; Neoplasm Invasiveness; Proto-Oncogene Proteins c-kit; metabolism; Signal Transduction; Stem Cell Factor; metabolism; Urinary Bladder Neoplasms; metabolism; pathology
- From: Journal of Southern Medical University 2014;34(4):507-510
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of SCF/c-Kit signaling in the invasion of bladder cancer T24 cells.
METHODSWestern blotting was used to detect the expression of c-Kit and PI3K pathway activation stimulated by stem cell factor (SCF) in T24 cells. The invasiveness of T24 cells before and after SCF stimulation and Wortmannin (aspecific PI3K inhibitor) treatment was evaluated using Transwell invasion assay (direct and indirect counting methods).
RESULTST24 cells expressed c-Kit protein and showed obvious Akt phosphorylation after stimulation with SCF (1 ng/ml) for 24 h. Compared to the control group, SCF stimulation (1 ng/ml) caused a greater number of T24 cells to migrate through the polycarbonate film (P<0.01), and this effect was blocked by the application of Wortmannin before the stimulation.
CONCLUSIONSCF/c-Kit signaling promotes the invasiveness of T24 cells, and this effect is mediated by the PI3K pathway.
