Role of Sam68 in proliferation, invasion and migration of colorectal cancer cells in vitro.
- Author:
Liyang WANG
1
;
Yanmei CUI
;
Wenting LIAO
;
Side LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; metabolism; Cell Cycle Proteins; Cell Line, Tumor; Cell Movement; Cell Proliferation; Colorectal Neoplasms; metabolism; pathology; DNA-Binding Proteins; metabolism; Humans; RNA-Binding Proteins; metabolism
- From: Journal of Southern Medical University 2014;34(4):546-551
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of Sam68 (Src-associated substrate during mitosis 68 kD) in the occurrence and development of colorectal cancer.
METHODSColorectal cancer cell lines with stable Sam68 over-expressing and low Sam68 expression were established to test the effect of Sam68 in the proliferation, invasion, and migration of the cancer cells using colony formation, MTT and Transwell assays.
RESULTSSW480 and Ls174t colorectal cell lines over-expressing Sam68 showed significantly enhanced cell proliferation, invasion and migration (P<0.05). Conversely, the low Sam68 expression in SW620 and HCT116 colorectal cell lines significantly suppressed the cell proliferation, invasion and migration (P<0.05).
CONCLUSIONThe expression of Sam68 can promote the proliferation, invasion and migration of colorectal cancer cells lines in vitro.
