Peptidic HIV-1 fusion inhibitor VIR576 as a potential dual- functional microbicide inhibits antigen-specific CD4(+) T-cell activation.
- Author:
Minmin LI
1
;
Ruitao ZHANG
;
Yiping HU
;
Jianjun LI
;
Shibo JIANG
;
Xiaojuan LI
;
Shuwen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CD3 Complex; CD4-Positive T-Lymphocytes; drug effects; HIV Fusion Inhibitors; pharmacology; HIV-1; Lymphocyte Activation; drug effects; Mice; Mice, Transgenic; Ovalbumin; Peptide Fragments; pharmacology; alpha 1-Antitrypsin; pharmacology
- From: Journal of Southern Medical University 2014;34(5):597-602
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo observe if VIR576, an 20-mer peptide derived from the C-proximal subfragment of a1-antitrypsin (a1-AT) which inhibits human immunodeficiency virus type 1 (HIV-1) entry into the target cells by interacting with fusion peptide (FP), can also directly inhibit CD4(+) T cell activation in vitro.
METHODSSplenocytes isolated from DO11.10 OVA Tg mice were stimulated with ovalbumin or concanavalin A to test the effects of VIR576 on antigen-specific or non-antigen-specific T cell activation. Both primary CD4(+)CD25(-) T cells from DO11.10 mice and CD4(+) T cell line A2b were activated with specific antigens to evaluate the effects of VIR576.
RESULTSVIR576 inhibited antigen-specific splenocyte activation but had no significant effect on non-antigen-specific T-cell activation, which bypassed the crosstalk between the CD3-signaling complex and TCR. We furthermore observed that VIR576 could also down-regulate antigen-specific CD4(+) T-cell activation.
CONCLUSIONSGiven the high susceptibility of activated CD4(+) T cells in the mucosa to HIV-1 infection, the inhibitory effects of VIR576 on both HIV entry into the target cells and CD4(+) T-cell activation suggest the potential of VIR576 as a microbicide for prevention of sexual transmission of HIV.
