Effects of bone marrow-derived mast cells on expressions of type II collagen and glycosaminoglycan in co-cultured chondrocytes.
- Author:
Qingqing OUYANG
1
;
Jinjun ZHAO
;
Min YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; Cells, Cultured; Chondrocytes; cytology; Coculture Techniques; Collagen Type II; metabolism; Glycosaminoglycans; metabolism; Mast Cells; cytology; Mice
- From: Journal of Southern Medical University 2014;34(5):669-673
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of the bone marrow-derived mast cells (BMMCs) on the expression of type II collagen and glycosaminoglycan (GAG) in chondrocytes co-cultured with BMMCs.
METHODSPrimarily cultured mouse BMMCs at 4 weeks and the second passage of chondrocytes were plated in a Transwell co-cultured system at a ratio of 1:10 in the presence or absence of sodium cromoglycate (DSCG) or compound 48/80 (C48/80). The chondrocytes were harvested and lysed for detecting type II collagen expression with ELISA and Western blotting and GAG expression using 1,9 dimethylmethylene blue (DBM).
RESULTSAfter a 24-hour culture, the chondrocytes co-cultured with BMMCs showed similar expression levels of type II collagen and GAG to the control group regardless of the presence of DSCG (P>0.05). Compared with chondrocytes cultured alone or with BMMCs, the co-cultured chondrocytes in the presence of C48/80 showed significantly lower expressions of type II collagen and GAG (P<0.01). Such results did not vary significantly as the culture time was extended to 48 h.
CONCLUSIONC48/80-activated BMMCs can reduce the expression of type II collagen and GAG in chondrocytes in the co-culture system.