Small interfering RNA-mediated COX-2 gene silencing enhances chemosensitivity of KB/VCR cells by suppressing MDR-1 gene expression and P-glycoprotein activity.
- Author:
Xianchao MO
1
;
Weizhong LI
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter, Sub-Family B; genetics; metabolism; Cyclooxygenase 2; genetics; metabolism; Drug Resistance, Neoplasm; genetics; Gene Expression Regulation, Neoplastic; Gene Silencing; Humans; KB Cells; RNA, Messenger; RNA, Small Interfering; Transfection; Vincristine
- From: Journal of Southern Medical University 2014;34(5):718-722
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of small interfering RNA (siRNA)-mediated COX-2 gene silencing in enhancing the chemosensitivity of KB/VCR cell lines.
METHODSKB/VCR cells were trasnfected with COX-2 siRNA were examined for expressions of COX-2 and MDR-1 mRNAs with RT-PCR and for Rho-123 accumulation using flow cytometry. MTT assay was used to analyze the proliferation of the transfected KB/VCR cells.
RESULTSCompared with the negative and blank control groups, COX-2 siRNA transfection resulted in significant growth inhibition of KB/VCR cells exposed to vincristine (P<0.01), down-regulated the expressions of COX-2 and MDR-1 mRNAs, and obviously increased Rho-123 accumulation in KB/VCR cells.
CONCLUSIONCOX-2 gene silencing can enhance the chemosensitivity of KB/VCR cells to vincristine, the mechanism of which may involve down-regulated MDR-1 gene expression and inhibition of P-glycoprotein activity.
