Establishment of a hepatic fibrosis model induced by diethylnitrosamine in zebrafish.
- Author:
Kunyuan WANG
1
;
Li LIU
;
Wencong DAI
;
Xiaohui CHEN
;
Xinchun ZHENG
;
Jinlin HOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Diethylnitrosamine; Disease Models, Animal; Liver Cirrhosis; chemically induced; Zebrafish
- From: Journal of Southern Medical University 2014;34(6):777-782
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a zebrafish model of liver fibrosis via diethylnitrosamine (DEN)-induced liver injury.
METHODSA total of 120 wild-type 3-month-old zebrafish were randomly divided into DEN-treated group and control group. The survival rate and behavioral changes of each group were observed. After treatment with DEN for 2, 4, and 6 weeks, liver index was measured, and liver fibrosis was evaluated with HE staining, Gomori staining and Sirius red staining.
RESULTSNo obvious behavioral change was observed in DEN-treated group during the experiment. Compared with that in control group, the liver index of zebrafish in DEN-treated group showed no significantly changes at the time points of observation. Proliferation of reticulate fibers was found in 30% of zebrafish treated with DEN for 4 weeks, and the rate increased to 80% at 6 weeks when reticulate fibers and collagen fibers actively proliferated to result in fiber collapse and formation of fibrotic nodules.
CONCLUSIONA stable zebrafish liver fibrosis model was successfully established by inducing liver damage to facilitate studies of the pathogenesis of liver fibrosis and screening therapeutic drugs.
