Diagnostic values of salivary versus and plasma microRNA-21 for early esophageal cancer.
- Author:
Minhua YE
1
;
Penghui YE
;
Weizhu ZHANG
;
Jiaqi RAO
;
Zijun XIE
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; blood; chemistry; Esophageal Neoplasms; blood; diagnosis; Humans; MicroRNAs; blood; Plasma; chemistry; Real-Time Polymerase Chain Reaction; Saliva; chemistry; Sensitivity and Specificity
- From: Journal of Southern Medical University 2014;34(6):885-889
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the diagnostic value of salivary and plasma miR-21 in patients with esophageal cancer (EC).
METHODSTotal RNA was extracted from saliva and plasma samples from 50 stage I and 50 stage II patients with EC and 50 healthy controls for measurement of miR-21 levels using qPCR. The diagnostic values of salivary and plasma miR-21 levels were assessed for stage I, stage II, and stage I+II EC.
RESULTSSalivary and plasma miR-21 were significantly higher in the EC patients than in the control group. The diagnostic sensitivities of plasma miR-21 for stage I, stage II, and stage I+II EC were 96%, 64% and 97%, with specificities of 44%, 84%, and 56%, respectively; the sensitivities of salivary miR-21 were 90%, 88%, and 89%, respectively, with the same specificities of 64%. Regardless of EC staging, the expression of plasma miR-21 showed a significant positive correlation with that of salivary miR-21, and their diagnostic values were comparable.
CONCLUSIONBoth salivary and plasmatic miR-21 can be sensitive biomarkers for EC, and salivary miR-21 detection has the potential to replace plasma detection for EC diagnosis.
