Effects of serum enatninine Gumibao (Chinese character: see text) on the aroliferation and differentiation of osteoblast induced by dexamethasone.
- Author:
Yi SONG
;
Hong JIAN
;
Dao-fang DING
;
Ling-hui LI
;
Guo-qing DU
;
Jin-tao LIU
;
Hong-sheng ZHAN
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Collagen Type I; analysis; Dexamethasone; pharmacology; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; pharmacology; Female; Osteoblasts; cytology; drug effects; physiology; Proliferating Cell Nuclear Antigen; analysis; Rats; Rats, Sprague-Dawley
- From: China Journal of Orthopaedics and Traumatology 2014;27(8):668-672
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of serum containing Gumibao (Chinese character: see text) on the proliferation and differentiation of osteoblast induced by dexamethasone.
METHODSOsteoblasts were extracted from skulls in newly born (within 24 hours) SD rats, and digested with collagenase. The first passage of cells were used for experiments. Cells were cultured in the medium containing different concentrations of dexamethasone (0, 10(-8), 10(-7), 10(-6), 10(-5) ,10(-4) mol/L). Alkaline phosphatase staining were carried out after 1 week and numbers of mineralized nodes with alizarin red staining were observed after 3 weeks. Accordingly, following the treatment of 10(-5) mol/L dexamethasone for 1 week, cells were cultured in the medium with serum containing Gumibao (Chinese character: see text). One week after Cumibao (Chinese character: see text) treatment, cells were stained with Alkaline phosphatase and collagen I and PCNA were examined by Western-blot. However, the observation of numbers of mineralized nodes with alizarin red stain required one more week.
RESULTSHigh concentration of dexamethasone could inhibit the expression of PCNA, collagen I, alkaline phosphatase and reduce the number of mineralized nodes of osteoblast, while serum containing Gumibao (Chinese character: see text) could reverse the inhibition.
CONCLUSIONHigh concentration of dexamethasone could inhibit the proliferation and differentiation of osteoblastic cells, while serum containing Gumibao (Chinese character: see text) could reverse the inhibition.