Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells.
- Author:
Li-Ping CAO
1
;
Guo-Ping DING
;
Ri-Sheng QUE
;
Shu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Breast Neoplasms; chemistry; metabolism; Carbon Dioxide; administration & dosage; Cell Line, Tumor; Cell Proliferation; drug effects; Cell Survival; drug effects; Enzyme Activation; drug effects; Hydrogen-Ion Concentration; Intracellular Fluid; chemistry; Phosphoprotein Phosphatases; metabolism; Pneumoperitoneum, Artificial; methods; Protein Kinase C; metabolism; Protein Phosphatase 2; Rats; Signal Transduction; drug effects
- From: Journal of Zhejiang University. Science. B 2005;6(7):650-655
- CountryChina
- Language:English
-
Abstract:
OBJECTThe authors studied the influence of CO(2) pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation.
METHODThey set up a simulation of pneumoperitoneum under different CO(2) pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated.
RESULTWhen the pressure of CO(2) pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g.min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g.min) respectively. After 1 week, the cancer cells concentration was 2.15 x 10(5), 2.03 x 10(5), 2.20 x 10(5), 2.18 x 10(5) L(-1).
CONCLUSIONCO(2) pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.
