Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase.
- Author:
Hua YANG
1
;
Shu ZHENG
;
Laurent MEIJER
;
Shi-min LI
;
Sophie LECLERC
;
Lin-lin YU
;
Jin-quan CHENG
;
Su-zhan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Cycle Proteins; antagonists & inhibitors; metabolism; Cell Line, Tumor; Cyclin-Dependent Kinases; metabolism; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; analysis; chemistry; pharmacology; Humans; Lethal Dose 50; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; drug therapy; enzymology; pathology; Medicine, Chinese Traditional; methods; Mitosis; drug effects; Phytotherapy; methods; Plants, Medicinal; chemistry; cdc25 Phosphatases; antagonists & inhibitors; metabolism
- From: Journal of Zhejiang University. Science. B 2005;6(7):656-663
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo screen and evaluate the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 phosphatase.
METHODSThe affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes are used as the cell cycle-specific targets for screening the antimitotic constituents. We tested 9 extracts isolated from the Chinese medicinal herbs and vegetables including the agents currently used in cancer treatment by measuring the inhibition of Cdc25A phosphatase and Cdc2 kinase activity. The antitumor activity of the extracts was also evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry.
RESULTSCdc25A inhibitory activity and antitumor activity are detected in the extracts isolated from three Chinese medicinal herbs Agrimona pilosa; Herba solani lyrati; Galla chinesis.
CONCLUSIONWe found three extracts isolated from Chinese medicinal herbs have potential inhibitory activity of Cdc25 phosphatase using a highly specific mechanism-based screen assay for antimitotic drug discovery.
