Role of short haipin RNA targeting vascular endothelial growth factor-C on biological characteristics of human breast cancer cell MCF-7.
- Author:
Xiao-bin XIE
1
;
Jie LONG
;
Fang YANG
;
Ya-jie ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; Down-Regulation; Female; Genetic Vectors; Humans; Neoplasm Invasiveness; RNA Interference; RNA, Messenger; metabolism; RNA, Small Interfering; genetics; Recombinant Proteins; genetics; metabolism; Transfection; Vascular Endothelial Growth Factor C; genetics; metabolism
- From: Chinese Journal of Pathology 2009;38(7):472-476
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of short haipin RNA (shRNA) on expression of vascular endothelial growth factor C (VEGF-C) and proliferation and invasion behavior of human breast cancer cell MCF-7.
METHODSThe recombinant vector (pSIREN-VEGF-C) was transfected into the human breast cancer cell MCF-7 by liposome and the positive transfected cell clones were screened with puromycin. Expression of VEGF-C in MCF-7 cells after gene transfer was detected by real-time quantitative PCR and Western blot assay, respectively. Proliferation and invasion ability of transfected cells were analyzed by MTT and Transwell filter.
RESULTSThe expressions of VEGF-C mRNA and protein were decreased markedly compared with the control group after the transfection and the inhibitive ratio was 95% and 100% respectively (P<0.05). The proliferation of MCF-7 cells transfected by pSIREN-VEGF-C, measured with MTT assays, was significantly decended (P<0.05). The invasion ability of passing through the Transwell filter of MCF-7 cells transfected by pSIREN-VEGF-C were declined evidently (P<0.05).
CONCLUSIONThe recombinant vector (pSIREN-VEGF-C) have been proved not only to be effective and specific for down-regulation of VEGF-C, but also can inhibit the proliferation and invasion of MCF-7 cells significantly.
