Pilot study of differentiation of bone marrow derived mesenchymal stem cells into endothelial cells induced by B16 melanoma cells in vitro.
- Author:
Chun-Sheng NI
1
;
Nan ZHAO
;
Tao SUN
;
Xiu-Lan ZHAO
;
Xing-Hui WANG
;
Qiang GU
;
Bao-Cun SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; metabolism; Cell Differentiation; Cell Line, Tumor; Coculture Techniques; Endothelial Cells; cytology; metabolism; Factor VIII; metabolism; Male; Melanoma, Experimental; metabolism; pathology; Mesenchymal Stromal Cells; cytology; metabolism; Mice; Mice, Inbred C57BL; Pilot Projects; Vascular Endothelial Growth Factor A; metabolism; Vascular Endothelial Growth Factor Receptor-1; metabolism; Vascular Endothelial Growth Factor Receptor-2; metabolism
- From: Chinese Journal of Pathology 2009;38(6):402-407
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEBone-marrow derived mesenchymal stem cells (BMSC) have the potential to differentiate into endothelial cells. The aim of the study was to investigate the induction process of BMSC by B16 melanoma cells in vitro and to analyze the role of VEGF-a in the process.
METHODSA co-culture system containing BMSC and B16 melanoma cells based on transwell indirect model was established, and the induction process of BMSC by B16 melanoma cells was studied in vitro.
RESULTSBMSC were isolated from the bone marrow of C57 mice. BMSC expressed CD105, CD90, CD73, CD44 and CD166, and acquired expressin of endothelial phenotype markers including VEGFR-1, VEGFR-2 and Factor VIII after co-culture with B16 melanoma cells for 48 hours. The expression level of VEGFR-2 would be double and Factor VIII threefold more by extending the co-culture time to 72 hours. In the co-culture system, B16 melanoma cells also up-regulated the expression of VEGF-a.
CONCLUSIONSVEGF-a plays a significant role in the differentiation of BMSC into cells of endothelial phenotype, therefore, is important to tumor angiogenesis.
