Application of interphase fluorescence in situ hybridization in the diagnosis of lymphoma.
- Author:
Rui BI
1
;
Dong-mei LIN
;
Ya-ling HAN
;
Jun LU
;
Li-yan XUE
;
Shan ZHENG
;
Ming-rong WANG
;
Ning LÜ
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Child; Chromosomes, Human, Pair 11; Chromosomes, Human, Pair 14; Chromosomes, Human, Pair 18; Diagnosis, Differential; Female; Hodgkin Disease; diagnosis; genetics; pathology; Humans; In Situ Hybridization, Fluorescence; methods; Leukemia, Lymphocytic, Chronic, B-Cell; diagnosis; genetics; pathology; Lymphoma; classification; diagnosis; genetics; pathology; Lymphoma, Follicular; diagnosis; genetics; pathology; Lymphoma, Large B-Cell, Diffuse; diagnosis; genetics; pathology; Lymphoma, Mantle-Cell; diagnosis; genetics; pathology; Lymphoma, Non-Hodgkin; diagnosis; genetics; pathology; Male; Middle Aged; Translocation, Genetic; Young Adult
- From: Chinese Journal of Pathology 2009;38(11):733-738
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility of interphase FISH in the routine clinicopathological practice and its values in the differential diagnosis of lymphomas.
METHODSA total of 74 fresh tissue samples clinically suspicious of lymphoma were investigated by FISH using three probes including IgH/bcl-2, IgH/CCND1 and API2/MALT1, corresponding the translocation t(14;18), t(11;14) and t(11;18) respectively. The results of FISH were analyzed and compared with the histopathologic diagnosis.
RESULTSHistological evaluation eventually confirmed that there were 62 cases of lymphoma and 12 cases of reactive lymphoid processes. The translocations were detected in 7 cases in 62 cases of lymphoma: 3 demonstrated t(14;18) including 2 cases of follicular lymphomas and 1 nodular sclerosing Hodgkin lymphoma. Four cases had t(11;14) including mantle cell lymphoma (2 cases), follicular lymphoma (1 case) and small cell lymphoma (1 case). A lymphoid hyperplasia case showed detectable t(14;18). All 25 cases of DLBCL showed no evidence of t(14;18). Amplification or loss of regional genes was seen more often in malignant than in the benign cases.
CONCLUSIONInterphase FISH offers useful ancillary technology that plays an important role in differential diagnosis and classification of lymphoma.
