Transfection of exogenous gene: Lac Z into spinal cord of SD rats and its protein expression:an in vivo study.
- Author:
Qi LI
1
;
Bing-Fang ZENG
;
Jian-Guang XU
;
Wei-Qing KONG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Animals; Genetic Therapy; Lac Operon; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Spinal Cord; metabolism; Spinal Cord Injuries; therapy; Transfection; beta-Galactosidase; analysis
- From: China Journal of Orthopaedics and Traumatology 2012;25(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore a way of the gene therapy for acute spinal cord injury (ASCI) by vivo transfection of exogenous gene into spinal cord tissue.
METHODSTwenty-four rats of SD were divided into experiment group and control group (each group had 12 rats). After anaesthesia by abdominal cavity, lamina of thoracic vertebra of all rats were cut-open in prone position. Complex of plasmid and report gene-Lac Z, and plasmid without report gene-Lac Z were respectively injected into cavum subdural of SD rats of experiment group and control group by cation liposome (DOTAP) encapsulation. The rats were killed at the 2nd week after operation, spinal cord tissue of injected segments were detected by reverse transcription-polymerase chain raction (RT-PCR) and immunohistochemistry.
RESULTSIn experiment group, positive staining of beta-galactosidase can be clearly observed in neuron and glia cell of rat's spinal cord by immunohistochemistry detection. Lac Z mRNA in same area was also detected by RT-PCR. But, in control group, no above-mentioned positive results were found.
CONCLUSIONEffective transfection of exogenous gene in vivo into spinal cord is a new hot spot for treatment of SCI. Thus certain nerve growth factor imput partly area of spinal cord injury can promote central nerve regrowth and avoid early secondary injury.
