Detection of IgM antibody against hantavirus by chemiluminescent enzyme-linked immunosorbent assay.
- Author:
Wei-hong LI
1
;
Quan-fu ZHANG
;
Jian-dong LI
;
Shou-chun CAO
;
Yu-fang XING
;
Yan WEI
;
Chuan LI
;
Qin-zhi LIU
;
Mi-fang LIANG
;
Dong-lou XIAO
;
De-xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; blood; Antibody Specificity; Enzyme-Linked Immunosorbent Assay; methods; Hantavirus; immunology; Hemorrhagic Fever with Renal Syndrome; immunology; Humans; Immunoglobulin M; blood; Luminescent Measurements; methods
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(2):171-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a chemiluminescent enzyme-linked immunosorbent assay (CLEIA) for the detection of HTNV IgM antibody.
METHODSBlack solid 96 well microplate was coated with anti-human IgM-microantibody, HRP labeled HTNV recombinant nucleotide antigen was used as detection antigen, luminol-H2O2 was used as substrate, a CLEIA was established for the detection of HFRS patient serum IgM antibody and comparison of detection sensitivity, specificity, and stability were made between CLEIA and MacELISA.
RESULTSCorrelate coefficient of CLEIA with MacELISA is 0.97; detection sensitivity of CLEIA is 100 percent while that of MacELISA is 92.1 percent; detection specificity of CLEIA and MacELISA are both 100 percent; coefficient of variance for intra-assay and inter-assay of CLEIA are both less than 15 percent, which are comparative with MacELISA.
CONCLUSIONThe established method of CLEIA is a sensitive, selective, and stable method; it is suitable for the early detection of HFRS patient serum IgM antibody.
