Development of an enzyme-linked immunosorbent assay for the diagnosis of lethal avian influenza virus infection.
- Author:
Jian LU
1
;
Yu GUO
;
Zi LI
;
Yu-xi CAO
;
Qi WANG
;
Xiu-ping WANG
;
Jing-jing HUANG
;
Min WANG
;
Sheng-li BI
;
Yue-long SHU
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; blood; Enzyme-Linked Immunosorbent Assay; methods; Humans; Influenza A Virus, H5N1 Subtype; immunology; isolation & purification; Influenza, Human; diagnosis; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(2):180-181
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a convenient method for diagnosis of lethal avian influenza virus infection.
METHODSNinety-six-well microtiter plates were coated with 100 microl of H5N1 protein (0.1 microg/ml) diluted in coating buffer. These plates were used for enzyme-linked immunosorbent assay (ELISA).
RESULTSFour of the 23 suspected borderline cases of lethal avian influenza virus infection were positive by the ELISA, and all the healthy controls (n=234) were negative. These results were confirmed by hemagglutination-inhibition (HI) and neutralization tests, and results were totally consistent (100 percent) with each other among the three methods.
CONCLUSIONThe ELISA may be used in the screening for lethal avian influenza virus infection.
