Immune responses induced by recombinant adenovirus Ad5F35-LMP2 in rhesus monkeys.
- Author:
Wu-Ning MO
1
;
Ling ZHOU
;
Xiao-Bing WU
;
Zhan WANG
;
An-Zhou TANG
;
Guang-Wu HUANG
;
Shuang-Qing YU
;
Qi WANG
;
Shu-Qing YE
;
Hai-Jun DU
;
Yi ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviruses, Human; genetics; Animals; Cell Differentiation; Herpesvirus 4, Human; genetics; Immunity, Cellular; immunology; Immunization; methods; Macaca mulatta; Recombinant Fusion Proteins; genetics; immunology; Viral Matrix Proteins; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(3):226-228
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the specific cellular and humoral immune responses after immunization with recombinant adenovirus Ad5F35-LMP2 in rhesus monkeys.
METHODSSixteen rhesuses were immunized with Ad5F35-LMP2 through intra-muscular injection in three groups: high dosage group (1.5 x 10(10) TCID(50)/rhesus), medium dosage group (1.5 x 10(9)TCID(50)/rhesus), low dosage group (1.5 x 10(8)TCID50/rhesus) and the last group was control (PBS 4 ml/rhesus). They were totally immunized three times at intervals of one month. The EBV-LMP2 specific cellular immune responses were tested during the 0, 4, 8, 12 weeks by Elispot after immunization respectively. And the titers of anti-LMP2 antibody were tested by EIA at the same time.
RESULTSEBV-LMP2 specific cellular and humoral immune responses which were induced by recombinant adenovirus Ad5F35-LMP2 can be found in all the three dosage groups. The potency of immune responses was related with the dosage of immunization. Higher dosage elicited more potent immune response.
CONCLUSIONThe recombinant adenovirus Ad5F35-LMP2 could elicit LMP2 specific cellular and humoral immune responses in rhesus.
