Inhibitory effect of Chinese herbal medicine Xin-Kang oral liquid on replication of CVB3 RNA in Coxsackievirus B3 infected mice.
- Author:
Su-Jun WAN
1
;
Fan YANG
;
Xia-Zhen HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Coxsackievirus Infections; drug therapy; Drugs, Chinese Herbal; pharmacology; Enterovirus B, Human; drug effects; genetics; Mice; Myocarditis; drug therapy; virology; Phytotherapy; RNA, Viral; genetics; Reverse Transcriptase Polymerase Chain Reaction; Treatment Outcome; Virus Replication; drug effects; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(3):238-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the CVB3 RNA concentration in myocardial tissues and inhibitory effect of Chinese herbal medicine Xin-Kang oral liquid on viral RNA replication in Coxsackievirus B3 infected myocardium.
METHODSThe total RNA was extracted from the infected murine myocardium, the reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect enterovirus RNA concentration in infected mice with Coxsackievirus B3 gene band. We used "Gel works system" to scan the electrophoresis image to detect CVB3 gene band.
RESULTSThe mean concentration of myocardial CVB3 RNA of Xin-Kang oral liquid treated groups was markedly lower than that of virus control group (P>0.01), but the CVB3 RNA in myocardial tissues has not been destroyed by Xin-Kang oral liquid feed in different phase.
CONCLUSIONChinese herbal medicine Xin-Kang oral liquid could inhibit CVB3 RNA replication in myocardial tissue, but it did not destruct the virus.
