Correlation between load of polyomavirus and hemorrhagic cystitis.
- Author:
Chun-Rong TONG
1
;
Zhi-Ping TENG
;
Hong-Xing LIU
;
Peng CAI
;
Si-Kun MA
;
Cheng-Liang ZHEN
;
Yi ZENG
;
Dao-Pei LU
Author Information
- Publication Type:Journal Article
- MeSH: Capsid Proteins; genetics; Cystitis; diagnosis; etiology; virology; DNA, Viral; blood; genetics; urine; Hematopoietic Stem Cell Transplantation; adverse effects; Hemorrhage; diagnosis; etiology; virology; Humans; Polymerase Chain Reaction; methods; Polyomavirus; genetics; growth & development; Polyomavirus Infections; complications; virology; Viral Load
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(3):244-246
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the correlation between polyoma virus load and hemorrhagic cystitis after allogeneic stem cells transplantation for prevention of hemorrhagic cystitis.
METHODSBlood and urine specimens were collected from 40 healthy persons, 40 patient with stem cells transplantation and 20 cases complicated with hemorrhagic cystitis for determination of VP1 gene of polyomaviruses BK virus (BKV)/Jamestown Canyon virus (JCV) and simian virus 40 (SV40) by polymerase chain reaction (PCR) and EvaGreen stain fluorescence quantitative assay.
RESULTSIn the peripheral blood, all genes of BKV/JCV and SV40 were negative, while BKV gene in urine and blood from healthy persons and patient with stem cells transplantation was 15% (6/40) and 100% (40/40), respectively. The gene of JCV was positive in 10% (4/40) and 12% (5/40), the gene of SV40 was negative.
CONCLUSIONGenes of BKV and JCV was detectable in urine specimens of healthy persons and there was a correlation between the load of polyomavirus and incidence of hemorrhagic cystitis.
